The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 21, 2006
Filed:
Oct. 03, 2002
Masking of the background fluorescence and luminescence in the optical analysis of biomedical assays
Thomas Krahn, Hagen, DE;
Wolfgang Paffhausen, Leverkusen, DE;
Andreas Schade, Essen, DE;
Martin Bechem, Wuppertal, DE;
Delf Schmidt, Wuppertal, DE;
Thomas Krahn, Hagen, DE;
Wolfgang Paffhausen, Leverkusen, DE;
Andreas Schade, Essen, DE;
Martin Bechem, Wuppertal, DE;
Delf Schmidt, Wuppertal, DE;
Bayer Healthcare AG, Leverkusen, DE;
Abstract
In a process for the quantitative optical analysis of biological cells labelled with a fluorescent dye, the sensitivity of analytical detection can be considerably improved if a masking dye, which absorbs the excitation light for the fluorescent dye and/or its emission light is added to the solution surrounding the biological cells and/or if a separating layer permeable to the solution and absorbing and/or reflecting the excitation light or the emission light is applied to a layer of the biological cells at the bottom of a reaction vessel. This process can also be used for improving the sensitivity in the quantitative optical analysis of a luminescent biological cell layer. Analogously, these process principles can also be used in receptor studies for the masking of the interfering background radiation in the quantitative optical analysis of fluorescently or luminescently labelled reaction components.