The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 21, 2006

Filed:

Feb. 28, 2005
Applicants:

Matthew V. Lorenzi, Philadelphia, PA (US);

Thomas Spires, Monroe, NJ (US);

Dan You, Cranbury, NJ (US);

Roberto Weinmann, Princeton, NJ (US);

Marco Gottardis, Princeton, NJ (US);

Inventors:

Matthew V. Lorenzi, Philadelphia, PA (US);

Thomas Spires, Monroe, NJ (US);

Dan You, Cranbury, NJ (US);

Roberto Weinmann, Princeton, NJ (US);

Marco Gottardis, Princeton, NJ (US);

Assignee:

Bristol-Myers Squibb Company, Princeton, NJ (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
G01N 33/53 (2006.01); G01N 33/567 (2006.01);
U.S. Cl.
CPC ...
Abstract

Methods for determining whether a test agent inhibits a 17β-HSD3, the methods comprising: obtaining a recombinant host cell that expresses the 17β-HSD3; obtaining a reaction mixture comprising the 17β-HSD3, androstenedione, and the test agent; measuring the amount of testosterone in the reaction mixture by a scintillation proximity assay (SPA) using SPA beads conjugated with a testosterone-specific antibody, wherein when the amount of testosterone is lower in the presence of a test agent than in the absence of the test agent, the test agent is identified as an inhibitor of the 17β-HSD3.


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