The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 14, 2006
Filed:
Jan. 28, 2004
Nobuyoshi Esaki, Shiga, JP;
Tohru Yoshimura, Kyoto, JP;
Naoto Matsuyama, Osaka, JP;
Koji Mizuno, Osaka, JP;
Takayuki Bogaki, Hyogo, JP;
Toshitaka Minetoki, Hyogo, JP;
Kenji Ozeki, Hyogo, JP;
Nobuyoshi Esaki, Shiga, JP;
Tohru Yoshimura, Kyoto, JP;
Naoto Matsuyama, Osaka, JP;
Koji Mizuno, Osaka, JP;
Takayuki Bogaki, Hyogo, JP;
Toshitaka Minetoki, Hyogo, JP;
Kenji Ozeki, Hyogo, JP;
Alfresa Pharma Corproation, Osaka, JP;
Abstract
A method for detecting or measuring homocysteine in a sample includes the steps of (a) reacting a D-amino acid present in a sample with a D-amino acid converting enzyme to convert the D-amino acid into a substance that does not serve as a substrate of D-amino acid oxidase or D-amino acid acetyltransferase; (b) reducing homocysteine in the sample with a thiol compound; (c) reacting the reduced homocysteine with a methyltransferase and a methyl donor to newly produce D-amino acid; and (d) reacting the produced D-amino acid with the D-amino acid oxidase or the D-amino acid acetyltransferase in the presence of an SH reagent to produce hydrogen peroxide, and color-developing the produced hydrogen peroxide by using an oxidative color-developing agent.