The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 31, 2006
Filed:
Apr. 25, 2003
Ayrookaran J. Poulose, Belmont, CA (US);
Volker Schellenberger, Palo Alto, CA (US);
James T. Kellis, Jr., Portola Valley, CA (US);
Christian Paech, Daly City, CA (US);
Joanne Nadherny, San Francisco, CA (US);
Donald P. Naki, San Francisco, CA (US);
Katherine D. Collier, Redwood City, CA (US);
Robert M. Caldwell, Belmont, CA (US);
Ayrookaran J. Poulose, Belmont, CA (US);
Volker Schellenberger, Palo Alto, CA (US);
James T. Kellis, Jr., Portola Valley, CA (US);
Christian Paech, Daly City, CA (US);
Joanne Nadherny, San Francisco, CA (US);
Donald P. Naki, San Francisco, CA (US);
Katherine D. Collier, Redwood City, CA (US);
Robert M. Caldwell, Belmont, CA (US);
Genencor International, Inc., Palo Alto, CA (US);
Abstract
Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Protease variants are provided that contain substitutions of the amino acids at one or more residue positions so that the substitution alters the charge at that position to make the charge more negative or less positive compared to a precursor protease and thus the protease variant is more effective in a low detergent concentration system than a precursor protease. Also provided are protease variants containing substitutions of the amino acids at one or more residue positions so that the substitution alters the charge at that position to make the charge more positive or less negative compared to a precursor protease and thus the protease variant is more effective in a high detergent concentration system than a precursor protease. Protease variants are provided that contain substitutions of the amino acids at one or more residue positions so that the substitution alters the charge at that position to make the charge more negative or less positive compared to a precursor protease and thus the protease variant is more effective in a medium detergent concentration system than a precursor protease. Also provided are protease variants containing substitutions of the amino acids at one or more residue positions so that the substitution alters the charge at that position to make the charge more positive or less negative compared to a precursor protease and thus the protease variant is more effective in a medium detergent concentration system than a precursor protease. Further provided is a method of producing a protease variant that is more effective in a low detergent concentration system, medium detergent concentration system and high detergent concentration system than a precursor protease.