The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 7122337 B1

PDF
Full Text
Expired
Date of Patent:
Oct. 17, 2006

Filed:

Mar. 19, 2004
Applicant:

Gilles Gutierrez, Lyons, FR;

Inventor:

Gilles Gutierrez, Lyons, FR;

Assignees:

Texinfine SA, , FR;

Patrinove SC, , FR;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 1/00 (2006.01); C12N 1/12 (2006.01); C12P 1/00 (2006.01); C12Q 1/00 (2006.01); C12Q 1/02 (2006.01);
U.S. Cl.
CPC ...
Abstract

A method of determining calciotropic activity of a preparation of algae of the genusThe algae preparation may be in the form of a powder or an extract. The method consists of culturing human or animal osteoblasts in a culture medium containing calcium ions in a well plate. The preparation is added thereto and non-fixed calcium ions are eliminated in the culture medium. Further, the culture medium is acidified to destroy an extracellular matrix formed by the osteoblasts in the culture medium. Thereafter, filtering of the culture medium is carried out and then a concentration of fixed calcium ions in the osteoblasts is determined and compared with a calibration scale of a known activator of the fixation of calcium or in the presence of an inhibitor of the fixation of calcium or both.


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