The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 19, 2006
Filed:
Apr. 13, 2004
Rajender Singh Sangwan, Lucknow, IN;
Narayan Das Chaurasiya, Lucknow, IN;
Laxmi Narayan Misra, Lucknow, IN;
Payare Lal, Lucknow, IN;
Girish Chandra Uniyal, Lucknow, IN;
Neelam Singh Sangwan, Lucknow, IN;
Avdhesh Kumar Srivastava, Lucknow, IN;
Krishan Avtar Suri, Jammu, IN;
Ghulam Nabi Qazi, Jammu, IN;
Rakesh Tuli, Lucknow, IN;
Rajender Singh Sangwan, Lucknow, IN;
Narayan Das Chaurasiya, Lucknow, IN;
Laxmi Narayan Misra, Lucknow, IN;
Payare Lal, Lucknow, IN;
Girish Chandra Uniyal, Lucknow, IN;
Neelam Singh Sangwan, Lucknow, IN;
Avdhesh Kumar Srivastava, Lucknow, IN;
Krishan Avtar Suri, Jammu, IN;
Ghulam Nabi Qazi, Jammu, IN;
Rakesh Tuli, Lucknow, IN;
Council of Scientific & Industrial Research, New Delhi, IN;
Abstract
The present invention relates to an improved process of analytical and quantitative isolation of withaferin-A from(Sanskrit: Ashwagandha, English: winter cherry) and other plants and products therefrom, said method comprising steps of selecting most appropriate extraction medium composition, with extraction solvent system consisting of a defined admixture of water and alcohol in a range of proportion of alcohol within a narrow range of (methanol, ethanol etc.) (preferably 60% alcohol and 40% water) for the dry plant material and from 60% to 80% alcohol in the alcohol-water mixture for fresh plant material, the invented extractant composition leading to better/improved yields and accurate quantitative estimations of withaferin A existent in planta or in materia, selecting effective state of the tissue particularly use of the fresh materials improving yields, providing logistic benefits of isolations and diagnosis, and according several other advantages including technological, economic, preventing contaminations etc., the said method being valuable and advantageous for standardization of botanicals, metabolomic characterization of plants particularly chemotypes, analyzing metabolic transitions under physiological, genetical, environmental and biotic perturbations.