The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 12, 2006
Filed:
Apr. 18, 2002
Frits J. Fallaux, Leiderdorp, NL;
Robert C. Hoeben, Leiden, NL;
Alex Jan Van Der Eb, Oegstgeest, NL;
Abraham Bout, Moerkapelle, NL;
Domenico Valerio, Oegstgeest, NL;
Frits J. Fallaux, Leiderdorp, NL;
Robert C. Hoeben, Leiden, NL;
Alex Jan van der Eb, Oegstgeest, NL;
Abraham Bout, Moerkapelle, NL;
Domenico Valerio, Oegstgeest, NL;
Crucell Holland B.V., Leiden, NL;
Abstract
Presented are ways to address the problem of replication competent adenovirus in adenoviral production for use with, for example, gene therapy. Packaging cells having no overlapping sequences with a selected vector are suited for large scale production of recombinant adenoviruses. A system for use with the invention produces replication defective adenovirus. The system includes a primary cell containing a nucleic acid based on or derived from adenovirus and an isolated recombinant nucleic acid molecule for transfer into the primary cell. The isolated recombinant nucleic acid molecule is based on or derived from an adenovirus, has at least one functional encapsidation signal and at least one functional Inverted Terminal Repeat, and lacks overlapping sequences with the nucleic acid of the cell. Otherwise, the overlapping sequences would enable homologous recombination leading to replication competent adenovirus in the primary cell into which the isolated recombinant nucleic acid molecule is to be transferred.