The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 12, 2006
Filed:
Feb. 13, 2003
Bei Shan, Redwood City, CA (US);
R. Marc Learned, El Granada, CA (US);
M. Catherine Amaral, Lake Bluff, IL (US);
Steven L. Mcknight, Dallas, TX (US);
Fabienne C. DE LA Brousse, Dallas, TX (US);
Jin-long Chen, Hillsborough, CA (US);
Bei Shan, Redwood City, CA (US);
R. Marc Learned, El Granada, CA (US);
M. Catherine Amaral, Lake Bluff, IL (US);
Steven L. McKnight, Dallas, TX (US);
Fabienne C. de la Brousse, Dallas, TX (US);
Jin-Long Chen, Hillsborough, CA (US);
Amgen Inc., Thousand Oaks, CA (US);
Abstract
The invention provides methods and compositions for screening for pharmacological agents which regulate gene expression in mammals. An exemplary assay involves (a) contacting a mammalian cell comprising a knock-in mutant of a targeted native allele encoding a reporter of gene expression, wherein the expression of the reporter is under the control of the gene expression regulatory sequences of the native allele, with a candidate agent under conditions whereby but for the presence of the agent, the reporter is expressed at a first expression level; and, (b) measuring the expression of the reporter to obtain a second expression level, wherein a difference between the first and second expression levels indicates that the candidate agent modulates gene expression.