The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 05, 2006
Filed:
Mar. 04, 2002
Tony E. Godfrey, Pittsburgh, PA (US);
James D. Luketich, Pittsburgh, PA (US);
Siva Raja, Pittsburgh, PA (US);
Lori A. Kelly, Jeanette, PA (US);
Sydney D. Finkelstein, Pittsburgh, PA (US);
Tony E. Godfrey, Pittsburgh, PA (US);
James D. Luketich, Pittsburgh, PA (US);
Siva Raja, Pittsburgh, PA (US);
Lori A. Kelly, Jeanette, PA (US);
Sydney D. Finkelstein, Pittsburgh, PA (US);
University of Pittsburgh—of the Commonwealth System of Higher Education, Pittsburgh, PA (US);
Abstract
A method for balancing multiplexed PCR methods is provided. In the method, two or more sequential temporal PCR stages are used to effectively separate two or more PCR reactions in a single tube as an alternative to primer limiting to modulate the relative rate of production of a first amplicon by a first primer set and a second amplicon by a second primer set during the first and second amplification stages. Also provided are rapid RT-PCR methods that find particular use in intraoperative diagnoses and prognoses, for instance in diagnosing malignant esophageal adenocarcenoma by determining expression levels of carcinoembryonic antigen (CEA) in sentinel lymph nodes.