The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 22, 2006
Filed:
Apr. 16, 2001
Marcy E. Armstrong, Schwenksville, PA (US);
Robert D. Keys, Norristown, PA (US);
John A. Lewis, Norristown, PA (US);
Margaret A. Liu, Rosemont, PA (US);
William L. Mcclements, Doylestown, PA (US);
Marcy E. Armstrong, Schwenksville, PA (US);
Robert D. Keys, Norristown, PA (US);
John A. Lewis, Norristown, PA (US);
Margaret A. Liu, Rosemont, PA (US);
William L. McClements, Doylestown, PA (US);
Merck & Co., Inc., Rahway, NJ (US);
Abstract
Genes encoding herpes simplex virus type 2 (HSV-2) proteins were cloned into eukaryotic expression vectors to express the encoded proteins in mammalian muscle cells in vivo. Animals were immunized by injection of these DNA constructs, termed polynucleotide vaccines or PNV, into their muscles. In a DNA titration, it was found that a single immunization of ≧0.5 μg of (one) PNV, gave >90% seroconversion by ten weeks post immunization. Immune antisera neutralized both HSV-2 and HSV-1 in cell culture. When animals were challenged with HSV-2, significant (p<0.001) protection from lethal infection was achieved following PNV vaccination. DNA constructs may be full-length, truncated and/or mutated forms and may be delivered along or in combination in order to optimize immunization and protection from HSV infection.