The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 08, 2006
Filed:
May. 19, 2003
John C. Gerdes, Denver, CO (US);
Elaine Best, Fort Collins, CO (US);
Jeffery M. Marmaro, Aurora, CO (US);
John C. Gerdes, Denver, CO (US);
Elaine Best, Fort Collins, CO (US);
Jeffery M. Marmaro, Aurora, CO (US);
Applera Corporation, Foster City, CA (US);
Abstract
The present invention provides a two-step multiplex amplification reaction wherein the first step truncates the standard initial multiplex amplification round to 'boost' the sample copy number by only a 100–1000 fold increase in the target. Following the first step the product is divided into optimized secondary single amplification reactions, each containing one of the primer sets that were used previously in the first or multiplexed booster step. The booster step can occur using an aqueous target nucleic acid or using a solid phase archived nucleic acid. In particular, nucleic acid sequences that uniquely identifywere identified using the multiplex amplification method.