The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 01, 2006
Filed:
Feb. 14, 2002
Shanavaz Nasarabadi, Livermore, CA (US);
Richard G. Langlois, Livermore, CA (US);
Kodumudi S. Venkateswaran, Livermore, CA (US);
Shanavaz Nasarabadi, Livermore, CA (US);
Richard G. Langlois, Livermore, CA (US);
Kodumudi S. Venkateswaran, Livermore, CA (US);
The Regents of the University of California, Oakland, CA (US);
Abstract
Disclosed herein are two methods for rapid multiplex analysis to determine the presence and identity of target DNA sequences within a DNA sample. Both methods use reporting DNA sequences, e.g., modified conventional Taqman® probes, to combine multiplex PCR amplification with microsphere-based hybridization using flow cytometry means of detection. Real-time PCR detection can also be incorporated. The first method uses a cyanine dye, such as, Cy3™, as the reporter linked to the 5' end of a reporting DNA sequence. The second method positions a reporter dye, e.g., FAM, on the 3′ end of the reporting DNA sequence and a quencher dye, e.g., TAMRA, on the 5′ end.