The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 18, 2006
Filed:
Feb. 23, 2001
John A. Heyman, Rixensart, BE;
Gabor Szalai, West Columbia, SC (US);
Michael Felder, Columbia, SC (US);
Knut R. Madden, Carlsbad, CA (US);
John A. Heyman, Rixensart, BE;
Gabor Szalai, West Columbia, SC (US);
Michael Felder, Columbia, SC (US);
Knut R. Madden, Carlsbad, CA (US);
Invitrogen Corporation, Carlsbad, CA (US);
South Carolina Research Institute, Columbia, SC (US);
Abstract
A Vaccinia topoisomerase-adapted linker, which contains an oligonucleotide primer binding site of known sequence, useful for specifically joining the linker to the end of a polynucleotide of unknown sequence and allowing subsequent PCR amplification of the polynucleotide or DNA is provided. Kits containing the invention Vaccinia topoisomerase-adapted linker and one or more linker-specific oligonucleotides for annealing to the linker in PCR are also provided. In addition, the invention provides methods for using the invention linkers in linker-mediated PCR amplification procedures for isolation and optional sequencing of isolated PCR amplification products.