The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 18, 2006
Filed:
May. 04, 2004
Teresa Di Colandrea, Ames, IA (US);
Cherisa Meyer, Ames, IA (US);
Won-bin Young, Ames, IA (US);
James N. Higginbotham, Hendersonville, TN (US);
Mario Mautino, Ames, IA (US);
Charles J. Link, Jr., Ames, IA (US);
Teresa Di Colandrea, Ames, IA (US);
Cherisa Meyer, Ames, IA (US);
Won-Bin Young, Ames, IA (US);
James N. Higginbotham, Hendersonville, TN (US);
Mario Mautino, Ames, IA (US);
Charles J. Link, Jr., Ames, IA (US);
Newlink Genetics Corporation, Ames, IA (US);
Abstract
A method is described for the rapid identification and isolation of cells based on the presence or absence of an ectopically-expressed N-acetyllactosaminide 3-α Galactosyltransferase (αGT) enzyme for the production of αGalactosyl-(1,3)Galactosyl (αGal) epitopes on the surface of αGal-negative cells. These cells which are genetically modified to express the αGT enzyme and αGal epitopes on glycosylated lipids and proteins of the cell surface are then labeled via an antibody composition which recognizes and binds the αGal epitopes on the cell surface. Cells labeled with the anti-αGal antibody can be isolated by sorting via fluorescence activated cell sorting (FACS), or by magnetic panning techniques. This method is suitable for the rapid positive or negative selection of αGal-positive cells from within a population of αGal-negative cells without the need to expose cells to antibiotics for any period of time. In addition, the specification provides a method for the production and purification of anti-αGal antibodies from chicken egg yolk.