The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 11, 2006
Filed:
Jun. 12, 2002
Christopher W. Hollars, Brentwood, CA (US);
Thomas R. Huser, Livermore, CA (US);
Stephen M. Lane, Oakland, CA (US);
Rodney L. Balhorn, Livermore, CA (US);
Olgica Bakajin, San Leandro, CA (US);
Christopher Darrow, Pleasanton, CA (US);
Joe H. Satcher, Jr., Patterson, CA (US);
Christopher W. Hollars, Brentwood, CA (US);
Thomas R. Huser, Livermore, CA (US);
Stephen M. Lane, Oakland, CA (US);
Rodney L. Balhorn, Livermore, CA (US);
Olgica Bakajin, San Leandro, CA (US);
Christopher Darrow, Pleasanton, CA (US);
Joe H. Satcher, Jr., Patterson, CA (US);
The United States of America as represented by the United States Department of Energy, Washington, DC (US);
Abstract
A method and apparatus with the sensitivity to detect and identify single target molecules through the localization of dual, fluorescently labeled probe molecules. This can be accomplished through specific attachment of the taget to a surface or in a two-dimensional (2D) flowing fluid sheet having approximate dimensions of 0.5 μm×100 μm×100 μm. A device using these methods would have 10–10greater throughput than previous one-dimensional (1D) micro-stream devices having 1 μminterrogation volumes and would for the first time allow immuno- and DNA assays at ultra-low (femtomolar) concentrations to be performed in short time periods (˜10 minutes). The use of novel labels (such as metal or semiconductor nanoparticles) may be incorporated to further extend the sensitivity possibly into the attomolar range.