The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 20, 2006
Filed:
Sep. 28, 2001
Thoams Krahn, Hagen, DE;
Wolfgang Paffhausen, Leverkusen, DE;
Andreas Schade, Essen, DE;
Martin Bechem, Wuppertal, DE;
Delf Schmidt, Wuppertal, DE;
Thoams Krahn, Hagen, DE;
Wolfgang Paffhausen, Leverkusen, DE;
Andreas Schade, Essen, DE;
Martin Bechem, Wuppertal, DE;
Delf Schmidt, Wuppertal, DE;
Bayer Healthcare AG, Leverkusen, DE;
Abstract
In a process for the quantitative optical analysis of fluorescently labelled biological cellsa cell layer on a transparent support at the bottomof a reaction vesselis in contact with a solutioncontaining the fluorescent dyeThe sensitivity of analytical detection can be considerably improved if to the fluorescent dyealready present in addition a masking dyewhich absorbs the excitation lightfor the fluorescent dyeand/or its emission lightis added to the solutionand/or if a separating layerpermeable to the solution and absorbing and/or reflecting the excitation lightor the emission lightis applied to the cell layer at the bottomThis process can also be used for improving the sensitivity in the quantitative optical analysis of a luminescent biological cell layer. The separating layermust in this case be composed such that it has a high power of reflection for the luminescent light. Analogously, these process principles can also be used in receptor studies for the masking of the interfering background radiation in the quantitative optical analysis of fluorescently or luminescently labelled reaction components. In this case, a receptor layerat the bottomof a reaction vesselis in contact with a solution (supernatant) in which a fluorescent or luminescent ligandis dissolved. The sensitivity and accuracy of the analytical detection can be considerably improved here if a masking dyewhich absorbs the excitation lightfor the fluorescent dye and/or its emission light or (in the case of luminescent ligands) the luminescent light is added to the supernatantInstead of the masking dye in the solutionor optionally as an additional measure, a separating layerpermeable to the solutionand absorbing and/or reflecting the excitation lightand/or the emission light or the luminescent light can be applied to the cell or receptor layerat the bottom