The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 13, 2006
Filed:
Jan. 13, 1999
Yukoh Hiei, Iwata-gun, JP;
Toshihiko Komari, Iwata-gun, JP;
Yukoh Hiei, Iwata-gun, JP;
Toshihiko Komari, Iwata-gun, JP;
Japan Tobacco Inc., Tokyo, JP;
Abstract
The invention relates to a method for transforming a monocotyledonous plant. The time required from transformation to regeneration of a plant is shorter using the inventive method so that the frequency of emergence of mutants is smaller than the conventional methods. The inventive method may be generally applied even to the plants for which a regeneration method from a protoplast to a plant has not been established, and with which the preparation of the material to be subjected to the method is easy. That is, the present invention provides a method for transforming a monocotyledonous plant, comprising contacting a cultured tissue of said monocotyledonous plant during dedifferentiation thereof obtained by culturing an explant on a dedifferentiation-inducing medium for less than 7 days with a bacterium belonging to the genuscontaining a super binary vector having the virulence region of a Ti plasmid, left and right border sequences of T-DNA of a Ti plasmid or an Ri plasmid of a bacterium belonging to the genus, and a desired gene located between said left and right border sequences.