The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 13, 2006

Filed:

Nov. 12, 2002
Applicants:

Richard A. Metz, Lawrenceville, NJ (US);

Bruce L. Frank, Langhorne, PA (US);

Debra M. Walther, Langhorne, PA (US);

Inventors:

Richard A. Metz, Lawrenceville, NJ (US);

Bruce L. Frank, Langhorne, PA (US);

Debra M. Walther, Langhorne, PA (US);

Assignee:

Other;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 15/63 (2006.01); C12N 15/88 (2006.01); C12N 15/87 (2006.01); A01N 43/04 (2006.01); C07H 21/02 (2006.01);
U.S. Cl.
CPC ...
Abstract

The invention concerns the introduction of predetermined genetic changes in target genes of a living cell by introducing an oligodeoxynucleotide encoding the predetermined change. The oligodeoxynucleotides are effective in animal, plant and bacterial cells. Specific end modifications that greatly increase the effectiveness of the oligodeoxynucleotides in bacteria are described. Surprisingly, unmodified oligodeoxynucleotides can be as effective in mammalian cells, including in vivo hepatocytes, as the modified nucleotides and can be as effective or more effective than chimeric oligonucleotides that consist of a mixture of deoxynucleotides and 2'-O-methyl ribonucleotides.


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