The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 25, 2006
Filed:
Dec. 07, 2001
John Carrino, San Diego, CA (US);
James Fan, San Diego, CA (US);
Robert P. Bennett, Encinitas, CA (US);
Jonathan D. Chesnut, Encinitas, CA (US);
Martin A. Gleeson, San Diego, CA (US);
Knut R. Madden, Carlsbad, CA (US);
John Carrino, San Diego, CA (US);
James Fan, San Diego, CA (US);
Robert P. Bennett, Encinitas, CA (US);
Jonathan D. Chesnut, Encinitas, CA (US);
Martin A. Gleeson, San Diego, CA (US);
Knut R. Madden, Carlsbad, CA (US);
Invitrogen Corporation, Carlsbad, CA (US);
Abstract
A method of generating a double stranded (ds) recombinant nucleic acid molecule covalently linked in both strands by contacting two or more ds nucleotide sequences with a topoisomerase under conditions such that both termini of at least one end of a first ds nucleotide sequence are covalently linked by the topoisomerase to both termini of at least one end of a second ds nucleotide sequence is provided. Also provided is a method for generating a ds recombinant nucleic acid molecule covalently linked in one strand, by contacting two or more ds nucleotide sequences with a type IA topoisomerase under conditions such that one strand, but not both strands, of one or both ends of a first ds nucleotide sequence are covalently linked by the topoisomerase. Compositions for performing such methods, and compositions generated from such methods also are provided, as are kits containing components useful for conveniently practicing the methods.