The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 18, 2006

Filed:

Mar. 30, 2001
Applicants:

Fiona Duffner, København, DK;

Reinhard Wilting, Farum, DK;

Kirk Schnorr, Holte, DK;

Inventors:

Fiona Duffner, København, DK;

Reinhard Wilting, Farum, DK;

Kirk Schnorr, Holte, DK;

Assignee:

Novozymes A/S, Bagsvaerd, DK;

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/00 (2006.01); C12P 19/34 (2006.01); C12N 15/00 (2006.01); C07H 21/02 (2006.01);
U.S. Cl.
CPC ...
Abstract

The present invention allows the screening of previously established genebanks or libraries by proxy for genes encoding secreted, partially secreted, or cell surface-displayed polypeptides of industrial interest, such as enzymes, receptors, cytokines, peptide hormones etc. that would not likely have been isolated using conventional screening assays. A method for isolating genes encoding secreted, partially secreted, or cell surface displayed polypeptides from existing gene libraries is described in which the endogenous secretion signal sequences are detected using an in vitro polynucleotide insertion reaction where the inserted polynucleotide comprises a promoter-less and secretion signal-less secretion reporter.


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