The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 21, 2006
Filed:
Mar. 17, 2000
Francis Barany, New York, NY (US);
Joseph P. Day, Pacifica, CA (US);
Robert P. Hammer, Baton Rouge, LA (US);
Donald E. Bergstrom, West Lafayette, IN (US);
Francis Barany, New York, NY (US);
Joseph P. Day, Pacifica, CA (US);
Robert P. Hammer, Baton Rouge, LA (US);
Donald E. Bergstrom, West Lafayette, IN (US);
Cornell Research Foundation,Inc., Ithaca, NY (US);
Purdue Research Foundation, West Lafayette, IN (US);
Board of Supervisors of Louisiana State University and Agricultural and Mechanical College, Baton Rouge, LA (US);
Abstract
The present invention provides a method for identifying one or more low abundance sequences differing by one or more single-base changes, insertions, or deletions, from a high abundance sequence in a plurality of target nucleotide sequences. The high abundance wild-type sequence is selectively removed using high fidelity polymerase chain reaction analog conversion, facilitated by optimal buffer conditions, to create a restriction endonuclease site in the high abundance wild-type gene, but not in the low abundance mutant gene. This allows for digestion of the high abundance DNA. Subsequently the low abundant mutant DNA is amplified and detected by the ligase detection reaction assay. The present invention also relates to a kit for carrying out this procedure.