The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 07, 2006
Filed:
Jun. 06, 1995
Sherrol H. Mcdonough, San Diego, CA (US);
Daniel L. Kacian, San Diego, CA (US);
Nanibhushan Dattagupta, San Diego, CA (US);
Diane L. Mcallister, San Diego, CA (US);
Philip W. Hammond, San Diego, CA (US);
Thomas B. Ryder, Escondido, CA (US);
Sherrol H. McDonough, San Diego, CA (US);
Daniel L. Kacian, San Diego, CA (US);
Nanibhushan Dattagupta, San Diego, CA (US);
Diane L. McAllister, San Diego, CA (US);
Philip W. Hammond, San Diego, CA (US);
Thomas B. Ryder, Escondido, CA (US);
Gen-Probe Incorporated, San Diego, CA (US);
Abstract
A method, composition and kit for synthesizing multiple copies of a target nucleic acid sequence autocatalytically under conditions of substantially constant temperature, ionic strength, and pH are provided in which multiple RNA copies of the target sequence autocatalytically generate additional copies using a mixture of blocked and unblocked primers and/or promoter-primers to initiate DNA and RNA synthesis, preferably with reduced non-specific product formation. The invention is useful for generating copies of a nucleic acid target sequence for purposes that include assays to quantitate specific nucleic acid sequences in clinical, environmental, forensic and similar samples, cloning and generating probes.