The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 07, 2006
Filed:
Aug. 09, 2002
Michael R. Gambini, Bolton, MA (US);
Susan Atwood Stone, Legal Representative, Charlotte, VT (US);
Judith K. Atwood, Legal Representative, Redding, CT (US);
Eugene F. Young, Foster City, CA (US);
Edward J. Lakatos, Bethel, CT (US);
Anthony L. Cerrone, New Haven, CT (US);
Michael R. Gambini, Bolton, MA (US);
Susan Atwood Stone, legal representative, Charlotte, VT (US);
Judith K. Atwood, legal representative, Redding, CT (US);
Eugene F. Young, Foster City, CA (US);
Edward J. Lakatos, Bethel, CT (US);
Anthony L. Cerrone, New Haven, CT (US);
Applera Corporation, Foster City, CA (US);
Abstract
An optical instrument monitors PCR replication of DNA in a reaction apparatus having a temperature cycled block with vials of reaction ingredients including dye that fluoresces in presence of double-stranded DNA. A beam splitter passes an excitation beam to the vials to fluoresce the dye. An emission beam from the dye is passed by the beam splitter to a CCD detector from which a processor computes DNA concentration. A reference strip with a plurality of reference emitters emit reference beams of different intensity, from which the processor selects an optimum emitter for compensating for drift. Exposure time is automatically adjusted for keeping within optimum dynamic ranges of the CCD and processor. A module of the beam splitter and associated optical filters is associated with selected dye, and is replaceable for different dyes.