The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 31, 2006

Filed:

Aug. 04, 2000
Applicants:

Joel S. Greenberger, Sewickley, PA (US);

David R. Hurwitz, Acton, MA (US);

Inventors:

Joel S. Greenberger, Sewickley, PA (US);

David R. Hurwitz, Acton, MA (US);

Assignee:

ALG Company, Marlborough, MA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A01N 63/00 (2006.01); A01N 1/02 (2006.01); A61K 48/00 (2006.01); C12N 5/02 (2006.01); C12N 15/09 (2006.01);
U.S. Cl.
CPC ...
Abstract

This invention relates to sequential methods of cryopreserving bone marrow stromal cells that are transfected and used for gene therapy by transplantation. These methods include the following steps in various orders: obtaining the cells, expanding the cells in culture, transfecting the cells, and cryopreserving the cells. With these methods, populations of bone marrow stromal cells can be acquired that are large enough to be useful in a number of therapies. Further, these large populations can be stored for extended periods of time for immediate use when needed.


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