The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 24, 2006
Filed:
Jul. 13, 2001
Jing Cheng, San Diego, CA (US);
Edward L. Sheldon, Iii, San Diego, CA (US);
Lei Wu, San Diego, CA (US);
James P. O'connell, San Diego, CA (US);
Jing Cheng, San Diego, CA (US);
Edward L. Sheldon, III, San Diego, CA (US);
Lei Wu, San Diego, CA (US);
James P. O'Connell, San Diego, CA (US);
Nanogen, Inc., San Diego, CA (US);
Abstract
The present invention comprises devices and methods for performing channel-less separation of cell particles by dielectrophoresis, DC high voltage-pulsed electronic lysis of separated cells, separation of desired components from crude mixtures such as cell lysates, and/or enzymatic reaction of such lysates, all of which can be conducted on a single bioelectronic chip. A preferred embodiment of the present invention comprises a cartridge () including a microfabricated silicon chip () on a printed circuit board () and a flow cell () mounted to the chip () to form a flow chamber. The cartridge () also includes output pins () for electronically connecting the cartridge () to an electronic controller. The chip () % includes a plurality of circular microelectrodes () which are preferably coated with a protective permeation layer. Specific cells from various cell mixtures were separated, lysed, and enzymatically digested on the chip.