The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 20, 2005
Filed:
Sep. 23, 2003
High-throughput turbidometric assay for screening inhibitors of protein disulfide isomerase activity
Applicants:
Shu-gui Huang, Fremont, CA (US);
Donna Oksenberg, Palo Alto, CA (US);
Roman Urfer, Belmont, CA (US);
Inventors:
Shu-Gui Huang, Fremont, CA (US);
Donna Oksenberg, Palo Alto, CA (US);
Roman Urfer, Belmont, CA (US);
Assignee:
AGY Therapeutics, Inc., South San Francisco, CA (US);
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q001/00 ;
U.S. Cl.
CPC ...
Abstract
Methods for performing an end-point assay of protein disulfide isomerase activity. The method may be based on the enzyme-catalyzed reduction of insulin in the presence of dithiothreitol; measuring the aggregation of reduced insulin chains at 650 nm; and using hydrogen peroxide as a stop reagent.