The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 29, 2005
Filed:
Nov. 20, 2001
Vladimir Knezevic, Gaithersburg, MD (US);
Michael R. Emmert-buck, Silver Spring, MD (US);
Galina Baibakova, Bethesda, MD (US);
Dan-paul Hartmann, Bethesda, MD (US);
Stephen M. Hewitt, Potomac, MD (US);
Capre Denise Mitchell, Winston Salem, NC (US);
Kevin Gardner, Montgomery Village, MD (US);
Vladimir Knezevic, Gaithersburg, MD (US);
Michael R. Emmert-Buck, Silver Spring, MD (US);
Galina Baibakova, Bethesda, MD (US);
Dan-Paul Hartmann, Bethesda, MD (US);
Stephen M. Hewitt, Potomac, MD (US);
Capre Denise Mitchell, Winston Salem, NC (US);
Kevin Gardner, Montgomery Village, MD (US);
20/20 GeneSystems, Inc., Rockville, MD (US);
The United States of America as represented by the Department of Health and Human Services, Washington, MD (US);
Abstract
The present disclosure is directed to devices, arrays, kits and methods for detecting biomolecules in a tissue section (such as a fresh or archival sample, tissue microarray, or cells harvested by an LCM procedure) or other substantially two-dimensional sample (such as an electrophoretic gel or cDNA microarray) by creating 'carbon copies' of the biomolecules eluted from the sample and visualizing the biomolecules on the copies using one or more detector molecules (e.g., antibodies or DNA probes) having specific affinity for the biomolecules of interest. Specific methods are provided for identifying the pattern of biomolecules (e.g., proteins and nucleic acids) in the samples. Other specific methods are provided for the identification and analysis of proteins and other biological molecules produced by cells and/or tissue, especially human cells and/or tissue. The disclosure also provides a plurality of differentially prepared and/or processed membranes that can be used in described methods, and which permit the identification and analysis of biomolecules.