The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 6955882 B1

PDF
Full Text
Expired
Date of Patent:
Oct. 18, 2005

Filed:

Jan. 23, 2003
Applicants:

Yves Hatzfield, Lille, FR;

Valérie Marie-noëlle Frankard, Brussels, BE;

Anne-marie Droual, Lille, FR;

Inventors:

Yves Hatzfield, Lille, FR;

Valérie Marie-Noëlle Frankard, Brussels, BE;

Anne-Marie Droual, Lille, FR;

Assignee:

CropDesign N.V., Ghent, BE;

Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q001/68 ; C12N015/00 ;
U.S. Cl.
CPC ...
Abstract

The invention provides a method that allows the construction of a chimeric and/or modified and/or reconstructed DNA molecule from two DNA fragments in a defined order and orientation, and to clone the molecule one step in a suitable vector using site specific recombination. No initial step of classical cloning via restriction enzymes is needed, in contrast to the classical recombination systems. This method allows the reliability of the recombination method for cloning with the flexibility of PCR to introduce modifications in the insert sequence. Moreover, this method allows the construction of chimerical DNA molecules associating two different elements, such as promoter-gene association or fusion proteins.


Find Patent Forward Citations

Hatzfield, Yves. (2005). Method for easy cloning and selection of chimeric dna molecules (U.S. Patent No. 6955882). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/6955882

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