The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 30, 2005
Filed:
Apr. 16, 2002
Xiaowu Liang, La Jolla, CA (US);
Andy Teng, Fullerton, CA (US);
Shizhong Chen, San Diego, CA (US);
Dongyuan Xia, Powell, OH (US);
Philip L. Felgner, Rancho Sata Fe, CA (US);
Xiaowu Liang, La Jolla, CA (US);
Andy Teng, Fullerton, CA (US);
Shizhong Chen, San Diego, CA (US);
Dongyuan Xia, Powell, OH (US);
Philip L. Felgner, Rancho Sata Fe, CA (US);
Gene Therapy Systems, Inc., San Diego, CA (US);
Abstract
A method for cloning a nucleic acid fragment into a vector by flanking the fragment with first and second adapter sequences, and contacting the fragment with the vector having sequences homologous to the first and second adapter sequences under conditions such that the nucleic acid fragment is incorporated into the vector by homologous recombination in vivo in a host cell. Additionally, a method for selecting for a successful transformation of a vector by a nucleic acid insert. Also, systems for cloning a nucleic acid fragment into a vector without at least one of a restriction enzyme, a ligase, a gyrase, a single stranded DNA binding protein, or other DNA modifying enzymes. Further, a kit for cloning a nucleic acid fragment into a vector.