The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 19, 2005

Filed:

Oct. 09, 2002
Applicants:

Kayo Hiyama, Kobe, JP;

Koichi Yamagata, Kobe, JP;

Makoto Ueda, Kakogawa, JP;

Kadzuki Nakabayashi, Kobe, JP;

Inventors:

Kayo Hiyama, Kobe, JP;

Koichi Yamagata, Kobe, JP;

Makoto Ueda, Kakogawa, JP;

Kadzuki Nakabayashi, Kobe, JP;

Assignee:

Sysmex Corporation, Hyogo, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q001/68 ; C12P019/34 ; C07H021/00 ; C07H021/02 ; C07H021/04 ;
U.S. Cl.
CPC ...
Abstract

A method of detecting a gene as an amplified product includes subjecting a solution containing the gene to a gene amplification reaction at a predetermined reaction temperature in the presence of a surfactant having a higher clouding point than the predetermined reaction temperature and detecting the gene as an amplified product, and a reagent kit for the method of detecting the gene as the amplified product utilizing the gene amplification includes a primer necessary for amplifying the gene, an enzyme for amplifying the gene, and a surfactant having a clouding point higher than the predetermined reaction temperature, wherein the primer is packed separately from the enzyme, and the surfactant is packed with the primer or the enzyme, or separately.


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