The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 31, 2005
Filed:
Apr. 04, 2002
J. Bruce Pitner, Durham, NC (US);
John Jacob Hemperly, Apex, NC (US);
Richard D. Guarino, Holly Springs, NC (US);
Magdalena Wodnicka, Durham, NC (US);
David T. Stitt, Freeland, MD (US);
Gregory J. Burrell, Red Lion, PA (US);
Timothy G. Foley, Jr., Forest Hill, MD (US);
Patrick Shawn Beaty, Selton, PA (US);
J. Bruce Pitner, Durham, NC (US);
John Jacob Hemperly, Apex, NC (US);
Richard D. Guarino, Holly Springs, NC (US);
Magdalena Wodnicka, Durham, NC (US);
David T. Stitt, Freeland, MD (US);
Gregory J. Burrell, Red Lion, PA (US);
Timothy G. Foley, Jr., Forest Hill, MD (US);
Patrick Shawn Beaty, Selton, PA (US);
Becton, Dickinson and Company, Franklin Lakes, NJ (US);
Abstract
The present invention relates to methods for detection and evaluation of metabolic activity of eukaryotic and/or prokaryotic cells based upon their ability to consume dissolved oxygen. The methods utilize a luminescence detection system which makes use of the sensitivity of the luminescent emission of certain compounds to the presence of oxygen, which quenches (diminishes) the compound's luminescent emission in a concentration dependent manner. Respiring eukaryotic and/or prokaryotic cells will affect the oxygen concentration of a liquid medium in which they are immersed. Thus, this invention provides a convenient system to gather information on the presence, identification, quantification and cytotoxic activity of eukaryotic and/or prokaryotic cells by determining their effect on the oxygen concentration of the media in which they are present.