The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 24, 2005
Filed:
Apr. 17, 1998
Joseph Fisher, Belmont, CA (US);
James Lorens, Menlo Park, CA (US);
Donald Payan, Hillsborough, CA (US);
Alexander Rossi, San Francisco, CA (US);
Joseph Fisher, Belmont, CA (US);
James Lorens, Menlo Park, CA (US);
Donald Payan, Hillsborough, CA (US);
Alexander Rossi, San Francisco, CA (US);
Rigel Pharmaceuticals, Inc., South San Francisco, CA (US);
Abstract
Described is a method for screening for alterations in exocytosis of a population of cells. The cells are sorted by a FACS machine by assaying for alterations in at least three of the properties selected from the group consisting of light scattering, fluorescent dye uptake, fluorescent dye release, annexin granule binding, surface granule enzyme activity, and the quantity of granule specific proteins. Methods for screening for bioactive agents capable of modulating exocytosis in a cell are also described. The methods provide for reduced background and increased specificity without increasing the time or steps involved in assaying for exocytosis.