The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 17, 2005
Filed:
Mar. 22, 2002
Rajender Singh Sangwan, Lucknow, IN;
Neelam Singh Sangwan, Lucknow, IN;
Bali Ram Tyagi, Lucknow, IN;
Avdhesh Kumar Srivastava, Lucknow, IN;
Usha Yadav, Lucknow, IN;
Rajender Singh Sangwan, Lucknow, IN;
Neelam Singh Sangwan, Lucknow, IN;
Bali Ram Tyagi, Lucknow, IN;
Avdhesh Kumar Srivastava, Lucknow, IN;
Usha Yadav, Lucknow, IN;
Council of Scientific and Industrial Research, New Delhi, IN;
Abstract
The present invention relates to a method for profiling of protein extract from hyper-acidic aerial parts of plant Scented Geranium (sp.), said method comprising steps of selecting effective extraction medium, with extraction medium consisting of 0.2 M sodium carbonate increasing percentage extract by about 600%, selecting effective medium to determine polypeptide pattern in said extract, with polypeptide pattern medium consisting of Sodium Carbonate ranging between 0.2 to 0.4 M, and 0.2 M Sodium Carbonate along with 50% dimethylsulfoxide, selecting effective media to determine enzyme multimolecular forms in said extract, with enzyme multimolecular forms media consisting of 0.2 M Tris-HCl buffer at pH 7.5, and optionally 50% dimethylsulfoxide; Sodium Carbonate at concentration ranging between 0.2 to 0.4 M and optionally 50% dimethylsulfoxide, and using biochemical and biophysical techniques comprising gel electrophoresis, spectrophotometry, centrifugation, protein estimation in said method to determine quantitative, qualitative, structural and functional facets of the proteins from said extract, and extraction compositions thereof.