The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 19, 2005
Filed:
Feb. 16, 1999
Transposable element-anchored, amplification method for isolation and identification of tagged genes
John A. Arbuckle, Woodbury, MN (US);
Timothy W. Fox, Des Moines, IA (US);
Robert B. Meeley, Des Moines, IA (US);
Benjamin A. Bowen, Berkeley, CA (US);
John A. Mcelver, Durham, NC (US);
John A. Arbuckle, Woodbury, MN (US);
Timothy W. Fox, Des Moines, IA (US);
Robert B. Meeley, Des Moines, IA (US);
Benjamin A. Bowen, Berkeley, CA (US);
John A. McElver, Durham, NC (US);
Pioneer Hi-Bred International Inc., Des Moines, IA (US);
Abstract
A method for the rapid isolation and identification of the DNA sequence of transposable element-tagged genes is provided. The method comprises a modified AFLP approach using a transposable element-anchored amplification to identify and clone an amplification product that is associated with a mutant phenotype. Once cloned, the amplification product of interest may be used to screen a cDNA library directly or may be sequenced and compared to available databases for sequence homology. A modification of this approach provides a method for the identification of the location of an additional type of insertion event into genomic DNA, more specifically transgene insertion into the genome of a host organism.