The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 05, 2005
Filed:
Apr. 10, 2001
Method for isolating and recovering target dna or rna molecules having a desired nucleotide sequence
Paul E. Nisson, Gaithersburg, MD (US);
Joel Jesse, Mt. Airy, MD (US);
Wu-bo LI, N. Potomac, MD (US);
Paul E. Nisson, Gaithersburg, MD (US);
Joel Jesse, Mt. Airy, MD (US);
Wu-bo Li, N. Potomac, MD (US);
Invitrogen Corporation, Carlsbad, CA (US);
Abstract
The invention generally concerns the use of amino acid denaturants for denaturing or separating double stranded nucleic acid molecules. More specifically, the present invention provides a method for the rapid isolation and recovery of a desired target DNA or RNA molecules from a mixture or library containing such molecules. The method involves the use of haptenylated probes and amino acid denaturants to select the desired molecules and eliminate the undesired library members from a sample. The invention also provides a method in which larger or full-length nucleic acid molecules can be isolated from the subpopulation of desired molecules.