The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 01, 2005
Filed:
May. 17, 1999
Nobuo Shimamoto, Mishima, JP;
Nobuo Shimamoto, Mishima, JP;
HSP Research Institute, Inc., Osaka, JP;
Abstract
The present invention relates in providing a process for recovering a soluble protein having a higher recovery rate by suppressing the aggregation of the protein when a denaturing agent is removed from the solubilization treatment solution containing a solubilized protein; and providing a process for removing a denaturing agent from the above solubilization treatment solution in which the aggregation of the protein is suppressed. In a process for recovering a soluble protein comprising the steps of removing a denaturing agent from a solubilization treatment solution prepared by solubilizing insoluble protein aggregates using the denaturing agent, the process of the present invention is characterized by subjecting the solubilization treatment solution to a treatment of removing the denaturing agent in the presence of a dihydric to tetrahydric, polyhydric alcohol at a concentration of 60 to 99% by volume, and thereafter diluting with a diluent solution the treatment solution obtained after the removal treatment. In a process for removing a denaturing agent from a solubilization treatment solution prepared by solubilizing insoluble protein aggregates using the denaturing agent, the process of the present invention is characterized by subjecting the solubilization treatment solution to a treatment of removing the denaturing agent in the presence of a dihydric to tetrahydric, polyhydric alcohol.