The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 01, 2005

Filed:

Feb. 28, 2002
Applicants:

Kevin J. Kayser, Woodstock, IL (US);

Ho-shin Park, Chicago, IL (US);

John J. Kilbane, Ii, Woodstock, IL (US);

Inventors:

Kevin J. Kayser, Woodstock, IL (US);

Ho-Shin Park, Chicago, IL (US);

John J. Kilbane, II, Woodstock, IL (US);

Assignee:

Gas Technology Institute, Des Plaines, IL (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N015/74 ; C12N001/21 ; C12P021/00 ;
U.S. Cl.
CPC ...
Abstract

A method for introducing and stabilizing heterologous and recombinant genes in a thermophilic host in which a characteristic gene defining a detectable host characteristic is inactivated or deleted from the thermophilic host, resulting in a modified thermophilic host expressing an absence of the detectable host characteristic. A DNA fragment of interest is inserted into the modified thermophilic host together with an intact characteristic gene, whereby the detectable host characteristic is restored to the thermophilic host, thereby enabling detection and confirmation of successful transformation using plasmid vectors and integration of the DNA fragment into the chromosome of the thermophilic host.


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