The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 25, 2005

Filed:

Feb. 04, 2000
Applicants:

Jacek Gaertig, Athens, GA (US);

Harry W. Dickerson, Jr., Athens, GA (US);

Theodore G. Clark, Ithaca, NY (US);

Inventors:

Jacek Gaertig, Athens, GA (US);

Harry W. Dickerson, Jr., Athens, GA (US);

Theodore G. Clark, Ithaca, NY (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A01N 6300 ; A01N 6500 ; C12P 2106 ; C12N 120 ; C12N 1500 ;
U.S. Cl.
CPC ...
Abstract

The ciliated protozoan Tetrahymena exemplifies a recombinant system for the expression of heterologous nucleic acids, preferably on the plasma membrane surface. Integration of a heterlogous nucleic acid into the β-tubulin gene, BTU1, of a paclitaxel-sensitivemutant that possesses btu1-1K350M β-tubulin allele allows screening for transformants using negative selection, as transformants have restored paclitaxel resistance. Transgenic ciliated protozoa of the invention can serve as live vaccines. For example, transgenic Tetrahymena expressingi-antigen protein on their surface are effective vehicles for vaccination of freshwater fish against infection by


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