The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 07, 2004
Filed:
Mar. 15, 2002
Bjorn Eggert Christensen, Bagsvaerd, DK;
Henrik Mollgaard, Lyngby, DK;
Svend Kaasgaard, Soborg, DK;
Jan Lehmbeck, Vekso, DK;
Novozymes A/S, Bagsvaerd, DK;
Abstract
The present invention relates to methods for producing a polypeptide of interest by (a) cultivating a mutant of a parent cell, wherein (i) the mutant comprises a first nucleic acid sequence encoding the polypeptide and a second nucleic acid sequence comprising a modification of at least one of the genes responsible for the biosynthesis or secretion of at least one toxin, and (ii) the mutant produces less of the toxin than the parent cell when cultured under the same conditions; and (b) isolating the polypeptide from the culture medium. Also, mutants of cells are provided, as well as methods for obtaining the mutant cells.