The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 26, 2004

Filed:

Jul. 10, 2002
Applicant:
Inventors:

Jing Zhang, St. Louis, MO (US);

John Dekker, Beverwijk, NL;

Antoinette C. van der Kuyl, Loosdrecht, NL;

Jolanda Maas, Amsterdam, NL;

Bob van Gemen, Almere, NL;

Assignee:

Primagen Holding B.V., Amsterdam, NL;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 ; C12P 1/934 ; C07H 1/900 ; C07H 2/100 ; C07H 2/104 ;
U.S. Cl.
CPC ...
C12Q 1/68 ; C12P 1/934 ; C07H 1/900 ; C07H 2/100 ; C07H 2/104 ;
Abstract

Methods for amplifying nucleic acid in a sample comprising providing the sample with a set of primers to enable synthesis of at least one nucleic acid strand complementary to at least part of the nucleic acid, wherein the set of primers comprises between 3-8 random bases, preferably clustered near the 3′ end of each primer in said set of primers. The methods of the invention are useful, for example, for determining whether samples derived from humans, mammals, poultry, or fish comprise nucleic acid of a pathogen. The methods are further suited for typing the pathogen and typing particular variants of said pathogen. The methods are also suited for the elucidation of the gene expression profile or genetic profile of cells.


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