The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 26, 2004

Filed:

Mar. 21, 2002
Applicant:
Inventor:

Yasushi Shigemori, Kisarazu, JP;

Assignee:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q 1/68 ; C12Q 1/44 ; C12Q 1/37 ;
U.S. Cl.
CPC ...
C12Q 1/68 ; C12Q 1/44 ; C12Q 1/37 ;
Abstract

The present invention provides methods of preparing a triple-stranded DNA molecule by forming a DNA and protein complex, which DNA has a linear double-stand molecule and a single-strand molecule where the linear single-stranded DNA is complementary to a 5′ end region of one strand in the linear double stranded DNA; and the protein component is a homogeneous recombinant protein and an exonuclease; and then the protein is removed from the complex; triple-stranded DNA; and methods of using the triple-stranded DNA to detect nucleic acid sequence.


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