The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 12, 2004
Filed:
Feb. 19, 2002
Ian Alexander Graham, York, GB;
Tony Robert Larson, Leeds, GB;
The University of York, York, GB;
Abstract
The invention provides a method for measuring an acyl coenzyme A (acyl CoA) ester or esters in a sample, comprising the steps of: a) forming a reaction mixture comprising the sample to be tested and a derivatizing agent; b) allowing the sample and said derivatizing agent to react, so as to form a fluorescent derivative(s) of any acyl CoA ester(s) present in the sample; and c) determining a level of said fluorescent derivative(s). Further provided is an assay for determining the level of an acyl coenzyme A (acyl CoA) ester or esters in a test sample, comprising the steps: a) obtaining the test sample from an organism to be tested; b) forming a reaction mixture comprising the sample to be tested and a derivatizing agent; c) allowing the sample and said derivatizing agent to react, so as to form a fluorescent derivative(s) of any acyl CoA ester(s) present in the sample; d) determining a level of said fluorescent derivative(s) ester(s); and e) comparing the level of said fluorescent derivative(s) in the test sample with a level of said fluorescent derivative(s) in a normal sample, such that a significant difference in the level of said fluorescent derivative(s) between the test sample and normal sample may be predictive of a fatty acid oxidation disorder in said organism. Also disclosed is a kit for use with the assay of the present invention.