The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 05, 2004
Filed:
May. 10, 2001
Henrik Clausen, Holte, DK;
Margarida Amado, La Jolla, CA (US);
Other;
Abstract
A novel gene defining a novel enzyme in the UDP-D-galactose: &bgr;-N-acetylglucosamine/&bgr;-N-acetylgalactosamine &bgr;1,3galactosyltransferase family, termed &bgr;3Gal-T5, with unique enzymatic properties is disclosed. The enzymatic activity of &bgr;3Gal-T5 is shown to be distinct from that of previously identified enzymes of this gene family. The invention discloses isolated DNA molecules and DNA constructs encoding &bgr;3Gal-T5 and derivatives thereof by way of amino acid deletion, substitution or insertion exhibiting &bgr;3Gal-T5 activity, as well as cloning and expression vectors including such DNA, cells tranfected with the vectors, and recombinant methods for providing &bgr;3Gal-T5. The enzyme &bgr;3Gal-T5 and &bgr;3Gal-T5-active derivatives thereof are disclosed, in particular soluble derivatives comprising the catalytically active domain of &bgr;3Gal-T5. Further, the invention discloses methods of obtaining &bgr;1,3galactosyl glycosylated saccharides, glycopeptides or glycoproteins by use of an enzymically active &bgr;3Gal-T5 protein or fusion protein thereof or by using cells stably transfected with a vector including DNA encoding an enzymatically active &bgr;3Gal-T5 protein as an expression system for recombinant production of such glycopeptides or glycoproteins. Also a method for the identification of DNA sequence variations in the &bgr;3Gal-T5 gene by isolating DNA from a patient, amplifying &bgr;3Gal-T5-coding exons by PCR, and detecting the presence of DNA sequence variation, are disclosed.