The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 14, 2004
Filed:
Mar. 28, 2002
Ramesh Ramchandra Bhonde, Pune, IN;
Anil Chatterji, Gora, IN;
Other;
Abstract
The present invention provides a process for large scale in vitro production of amoebocytes of Indian Horseshoe Crab ( ) ( ) from dissected gill flaps of , in Leibovitz L- culture medium concentration (2×), to provide enhanced generation of amoebocytes. The process comprises the steps of: dissecting gill flaps of ; washing the gill flaps with an antibiotic solution followed by alcohol; culturing the gill flaps in tissue culture plates of sterile saline on a Rocker platform; culturing further the gill flaps in Leibovitz L- culture medium (2×); purging the gill flaps with Tween 80 solution; and purging again the gill flaps with horseshoe crab serum, while keeping the gill flaps in the culture medium viable for 90 days by feeding with fresh medium at an interval of 10-15 days to enable the enhanced release of amoebocytes both within and outside the gill flaps.