The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 07, 2004

Filed:

Jul. 18, 2001
Applicant:
Inventors:
Assignee:

University of Geneva, Geneva, CH;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/37 ; C12P 2/106 ; C12N 1/114 ; C12N 1/102 ; C12N 1/108 ;
U.S. Cl.
CPC ...
C12Q 1/37 ; C12P 2/106 ; C12N 1/114 ; C12N 1/102 ; C12N 1/108 ;
Abstract

Polypeptides which have been separated by gel electrophoresis can be identified or characterized by a procedure which has two main stages. In the first stage the gel is digested with a polypeptide-cleaving agent such as an enzyme. This produces mainly large fragments which, in the second stage are electroblotted through a hydrophilic membrane on which is immobilized another polypeptide-cleaving reagent such as an enzyme onto a hydrophobic member, typically a membrane, e.g. of PVDF. The resulting fragments, usually peptides, are identified, preferably by MALDI-TOF MS, or a property may be determined, e.g. by interaction with an antibody.


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