The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 24, 2004
Filed:
Oct. 13, 2000
Jean-Philippe Deslys, Le Chesnay, FR;
Commissariat a l'Energie Atomique, Paris, FR;
Abstract
The invention concerns a method for purifying PrPres from a biological sample to be used for qualitative and/or quantitative determination of the PrPres in said sample. The method essentially consists in: (1) incubating, during 30 seconds to 2 hours, at a temperature less than 80° C., said biological sample with a buffer solution A comprising at least a surfactant in an amount ranging between a quarter and four times the weight of the biological sample and optionally a protease, to form a suspension S ; (2) adding to said suspension S resulting from (1) a buffer solution B in an amount sufficient for thinning said suspension, which buffer solution B consists of a solvent or mixture of solvents, which does not solubilize the PrPres and has a constant dielectric ranging between 10 and 25; (3) centrifuging the suspension S resulting from step (2); and (4) solubilizing said pellet in a buffer solution C comprising at least a surfactant and/or at least a chaotropic agent, at a temperature ranging between room temperature and 100° C.