The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 17, 2004

Filed:

May. 15, 1995
Applicant:
Inventors:

Ronald C. Lundquist, Minnetonka, MN (US);

David A. Walters, Groton, CT (US);

Assignee:

DeKalb Genetics Corporation, DeKalb, IL (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 1/500 ; A01H 5/04 ; A01H 5/00 ;
U.S. Cl.
CPC ...
C12N 1/500 ; A01H 5/04 ; A01H 5/00 ;
Abstract

This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs the Streptomyces bar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stablefs transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.


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