The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 22, 2004
Filed:
Sep. 17, 2001
Sanford A. Asher, Pittsburgh, PA (US);
Chad E Reese, Pittsburgh, PA (US);
University of Pittsburgh, Pittsburgh, PA (US);
Abstract
A novel method is disclosed wherein polymerized crystalline colloidal array (PCCA) chemical sensing materials are used to detect the presence of certain chemical species in high ionic strength solutions, such as bodily fluids. The PCCA sensors consist of a mesoscopically periodic array of colloidal particles polymerized into a hydrogel. The array of colloidal particles diffracts light in the visible spectral region due to the periodic spacing of the particles. The PCCA materials also contain molecular recognition components that bind to the chemical species being detected. The binding or the chelation of the chemical species of interest results in a Donnan potential that swells the hydrogel and in turn red shifts the diffracted light proportionately to the concentration of the chemical species. However, no significant red shift response may occur for PCCA chemical sensors in high ionic strength solutions containing chemical species of interest. Thus, the method of the present invention provides for incubating the PCCA chemical sensor in the sample solution, subsequently exposing the PCCA to a low ionic strength solution, such as pure water, and measuring the transient diffraction shift response of the PCCA upon the exposure to the low ionic strength solution. The non-chelated or non-bound ions (such as salt ions in high ionic strength solutions) diffuse out of the PCCA hydrogel more quickly than the chemical species ions bound to the molecular recognition component. Thus, the resulting transient PCCA diffraction red shift is proportional to the concentration of the chemical species in the sample solution. The present invention also discloses using the PCCA chemical sensors in an optrode for detecting certain chemical species in high ionic strength solutions, such as bodily fluids.