The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 15, 2004
Filed:
Sep. 05, 2001
Hideki Kambara, Hachioji, JP;
Guohua Zhou, Koganei, JP;
Kazunori Okano, Shiki, JP;
Masao Kamahori, Kokubunji, JP;
Hitachi, Ltd., Tokyo, JP;
Abstract
To provide a method and an apparatus for detecting DNA mutation without using electrophoresis, the presence or absence of specific sequence is detected by a method comprising a step of serially hybridizing a 5-base-long to 8-base-long short oligomer , which is capable of being engaged in the complementary strand extension reaction, and a long oligomer , which is capable of hybridizing with a target DNA but incapable of being engaged in the complementary strand extension reaction, with the target DNA ; a step of carrying out the complementary strand extension reaction starting from the short primer using four kinds of nucleic acid substrates and polymerase; and a step of detecting photo-emission, in which pyrophosphate produced as a reaction by-product of the complementary strand extension reaction is converted into ATP and the photo-emission reaction is carried out using an enzyme.