The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 18, 2004
Filed:
Apr. 26, 2000
John E. Sutton, Bellevue, NE (US);
Donn H. Vanden Bosch, Omaha, NE (US);
Transgenomic, Inc., Omaha, NE (US);
Abstract
A system for separating an aqueous stream of mixed polynucleotides into a series of length-based polynucleotide fractions and collecting one or more of the length-based polynucleotide fractions into separate containers. The system comprises a separation column containing separation media for separating an aqueous stream of mixed polynucleotides into a series of length-based polynucleotide fractions; a container including one or more single-sample containers; an ejection chamber having a separated sample inlet for receiving the length-based polynucleotide fractions, a waste outlet for discharging uncollected sample, and a capillary-sized fraction outlet positioned to discharge a selected length-based polynucleotide fraction into a single-sample container. The system also includes means for effecting discharge of a selected length-based polynucleotide fraction into the separate container. The means for effecting discharge of a selected length-based polynucleotide fraction into a separate container can include a puff valve having a pressurized gas inlet and a puff gas outlet and the ejection chamber can include a puff gas inlet communicating with the puff gas outlet, whereby activation of the puff valve will discharge a puff of gas into the ejection chamber and will effect discharge of sample through the fraction outlet; or the means can include a flow restriction actuator and flow restriction to increase liquid pressure in the ejection chamber. Computer controls and a method for fraction collection are also described.